Biol 202each question on the lab worksheet must be answered completely, thoroughly, in complete sentences and correctly in order to be considered for full credit if the question asks you to do research or find a source, a reputable, credible andor scholarly source citation must be. Oct 31, 2015 aseptic technique aseptic technique refers to a procedure that is performed under sterile conditions. Correctly sterilize and flame transfer instruments and tubes. Aseptic techniques and the transfer of microorganisms. The swab method a swab sample is taken from colonies to ensure the use of proper techniques and sterilization. Aseptic techniques aseptic technique refers to protocols and procedures you use to insure that no microbes contaminate your. Aseptic an environment or procedure that is free of contamination by pathogens bauman, robert w.
Aseptic technique is used for many procedures such as transferring cultures, inoculating media, and for performing a. A free powerpoint ppt presentation displayed as a flash slide show on id. The techniques are those that will be used frequently for studying microbes in the laboratory, and include those identified by the american society for microbiology in its recommendations for the microbiology laboratory core curriculum recommendations in. How to properly inoculate from broth to broth, broth to slant, and broth to plate tsb, nas, nap duration. Aseptic technique the media on which you culture desirable microorganisms will readily grow undesirable contaminants, especially molds and other types of fungus, and bacteria from your skin and hair. Aseptic technique is a set of principles and practices used by cell culture workers to reduce the presence of unwanted microorganisms or other cell lines in their cultures. The term often refers to those practices used to promote or induce asepsis in an operative field of surgery or medicine to prevent infection the goal of asepsis is to eliminate infection, not to achieve sterility. Aseptic transfer techniques or sterile transfer techniques are a series of procedures for working with live microorganisms and samples which may contain. Asepsis is a condition in which no living diseasecausing microorganisms are present. Here are some useful recommendations to help improve aseptic technique. Prevent contamination of the specific microorganism we are working with. If key parts become contaminated they can transfer microorganisms to the patient. Good aseptic technique is essential for successful longterm cell and tissue culture.
The main document is guidance for aseptic transfer processes in the nhs. The transfer of cultures between agar plates should be completed as. Its kind of superseded now that biological safety cabinets are commonplace as you can do work and keep things. Practicing aseptic technique with microbial cultures. In this weeks lab you will learn how to properly use this instrument. Semesterlong assessment of aseptic technique in microbiology. The purpose of this experiment is to gain knowledge of how to properly use aseptic techniques to transfer cultures, learning about cultured media and how to distinguish various types of microbial growths as well as what. We saw in the last chapter that microbiology is a discipline that is unusu ally dependent. January 14, 2020 by sagar aryal general aseptic techniques in microbiology laboratory.
Aseptic technique and the transfer of microorganisms theory. Use pourplating and spreadplating methods to determine the concentration of bacteria. Ppt aseptic technique powerpoint presentation free to. Microbiologist using aseptic techniques to spread bacteria culture on agar plate. All five methods incorporate aseptic technique, or procedures that maintain the sterility of experimental. Introduction to microbiology, sterile technique and micropipetting background. This chapter describes common laboratory procedures that can reduce the risk of culture contaminations sepsis, collectively referred as aseptic technique. Because bacteria are relatively simple organisms and are amenable to experimental manipulation, they have provided elegant systems for basic research. To have the opportunity to work with risk management techniques like haccp and rmc through practical workshops. Aseptic technique and the transfer of microorganisms. If bacteria are handled correctly, only the desired organisms will grow on transfer cultures.
Aseptic technique is essentially the backbone of microbiology. Aseptic technique and transfer of microorganisms last updated. Nonsterile supplies, media, and reagents, airborne particles laden with microorganisms, unclean incubators, and dirty work surfaces are all sources of biological contamination. Aseptic technique and culturing microbes lab 3 brittney case august 4th 20 section. Basic practical microbiology why microbiology matters. Semesterlong assessment of aseptic technique in microbiology labs. It avoids contamination by the microbes that are present in the air when you want to open a sterile tube or a tube of cultured microbes. Jan 11, 2014 aseptic technique the media on which you culture desirable microorganisms will readily grow undesirable contaminants, especially molds and other types of fungus, and bacteria from your skin and hair. Asepsis is the state of being free from diseasecausing microorganisms such as pathogenic bacteria, viruses, pathogenic fungi, and parasites. Aseptic technique in environmental science protocol. Choose from 500 different sets of aseptic technique microbiology flashcards on quizlet. Refer to your laboratory text for various methods of transferring microbial cultures.
Aseptic technique and streak plate method flashcards from celeste s. This concept map, created with ihmc cmaptools, has information related to. Aseptic transfer technique is important to prevent contamination of the culture being maintained as well as yourself. This manual has been written for a righthanded person. All five methods incorporate aseptic technique, or procedures that maintain the sterility of experimental materials. Thus, aseptic technique is an important concept to learn in the lab and in clinical settings.
Remove the tube cap and hold it between your little finger and ring finger of the hand holding the loop see figure 2. Sometimes these approaches form part of a drug companys total quality system. Asepsis covers all those procedures designed to reduce the risk of bacterial, fungal or viral contamination, using sterile instruments, sterile draping and the gloved no touch technique. Carry out aseptic technique for the removal and transfer of microorganisms for culturing. Aseptic technique aseptic technique refers to a procedure that is performed under sterile conditions. Lab 2 aseptic technique and transfer of microorganisms terminology, aerotolerant anaerobe definition cannot use oxygen for growth but tolerate it fairly well. The term often refers to those practices used to promote or induce asepsis in an operative field of surgery or medicine to prevent infection. Aseptic technique and transfer of microorganisms biology. Practicing aseptic technique with microbial cultures continued 2 21 linn cientiic nc all ights esere 6. Aseptic transfers growth medium sterilization microbiology. In a microbiology lab its essential to avoid contamination of sterile materials and isolated cultures with the bacteria and fungal. Aseptic technique microbiology medical specialties scribd. Aseptic technique an overview sciencedirect topics.
Asepsis covers all those procedures designed to reduce the risk of. Aseptic technique is a fundamental skill in microbiology, and has crucial applications in environmental research. If microbiological cultures are contaminated, the time, labor, and financial costs that would be required of a lab to clean up or replace the cultures, particularly rare isolates from unique environments, could be very high and prohibitive, and some samples may. Laura curtisclinical instructor or aseptic technique asepsis in the operating room patient surgical prep maintaining a sterile field using safe operative techniques. An introduction to microbiology, aseptic technique and safety preparation. Such techniques are essential for experiments that require growing cells. Module context this module introduces microbiological laboratory techniques to be used for analysis of coliforms bacteria can be used as indicators of pollution. Common among many experiments in microbiology are techniques involving the measurement and transfer of cultures containing bacterial cells or viral particles. A fundamental and important laboratory skill in the field of microbiology. Jan 10, 2017 it avoids contamination by the microbes that are present in the air when you want to open a sterile tube or a tube of cultured microbes. The purpose of this experiment is to gain knowledge of how to properly use aseptic techniques to transfer cultures, learning about cultured media and how to distinguish various. Aseptic techniques are designed to prevent unwanted microorganisms from contaminating either sterile materials or pure cultures. Introduction to microbiology, sterile technique and.
The simplest and most economical way to reduce contamination from airborne particles and aerosols e. The aseptic techniques control the opportunities for contamination of cultures. Aseptic technique is a set of routine measures that are taken to. Aseptic sterile technique used in microbiology laboratory. Aseptic technique, designed to provide a barrier between the microorganisms in the environment and the sterile cell culture, depends upon a set of procedures to reduce the probability of contamination from these sources. In the microbiology lab we use aseptic technique to. Produce nutrient agar na plates, na slants, and nutrient broths nbwhich will be used in.
This includes medical and laboratory techniques which deal with cultures and human cells and tissue for transplantation. Laboratory safety adapted from the american society of microbiology website. Aseptic technique and streak plate method microbiology bi127 with kryger at grand rapids community college studyblue. Two major strategies of aseptic work are described. Within microbiology, a shift is taking place from simple laboratory studies toward greater use of risk assessment and management 1. Successful cell culture depends heavily on keeping the cells free from contamination by microorganisms such as bacterial, fungi, and viruses. This method of preventing unwanted microorganisms from gaining access is termed aseptic technique. Pdf biology educators emphasize aseptic technique when teaching lab courses. Aseptic technique is a means of performing lab work that greatly reduces the risk of contamination. Objectives to demonstrate good aseptic technique in culture transfer or inoculation and in handling sterile materials. Aseptic transfer of cultures and sterile solutions 19. Testing sensitivity to antimicrobial substances 21. Aseptic technique is a fundamental and important laboratory skill in the field of microbiology.
Aseptic technique refers to a set of routine procedures done to prevent sterile solutions and cultures from becoming contaminated by unwanted microorganisms in the laboratory. To understand the fundamental mechanisms of contamination transfer from source to product and the protective measures in place to prevent product contamination. It is a fundamental skill for working in a microbiology laboratory. Exercise 6 and 7 pure culture technique and aseptic transfer of microorganisms and cultural characterization of bacteria. Explain how to sterilize it, and how to distribute it in different formats. Aseptic technique is very important in microbiology to ensure safety and.
Describe the different formats of media, plate, tube etc. Culturing and aseptic technique 100 points student name. Aseptic technique steps, purpose and importance of aseptic. Chart and diagram slides for powerpoint beautifully designed chart and diagram s for powerpoint with visually stunning graphics and animation effects. Sterile work area for labs with cell culture hoods. To prevent the access of microorganisms during the preparation and. Aseptic technique microbiology resource center truckee. The techniques are those that will be used frequently for studying microbes in the laboratory, and include those identified by the american society for microbiology in its recommendations for the microbiology laboratory core curriculum. Media and equipment selection sterile work area for labs with cell culture hoods. General aseptic techniques in microbiology laboratory basic. Manipulation of the tubes, plates and transfer tools requires patience and practice and is vital to success in the microbiology laboratory. Learn aseptic technique microbiology with free interactive flashcards.
Understand the concept of aseptic technique and its importance in the field of microbiology. Media, incubation, and aseptic technique objectives 1. Aseptic technique is a set of routine measures that are taken to prevent cultures, sterile media stocks, and other solutions from being contaminated by unwanted microorganisms i. Why is the aseptic technique important in microbiology.
This yellow cover document explains the need, outlines the risks and points the way to best practice. Bacteria represent the most widely distributed and metabolically diverse group of organisms. Microbiologists use aseptic technique for a variety of procedures such as transferring cultures, inoculating media, isolation of pure cultures, and for performing microbiological tests. Experiment 1 culture transfer technique purpose technique of aseptic removal and transfer of microorganisms for subculturing. Aseptic transfer of cultures and sterile solutions 19 preparing cultures for class use 19 part 2.
Feb 26, 2019 aseptic techniques refer to any method used to sterilize and maintain the sterility of an object or location. In the mid1800s, louis pasteur developed the techniques of aseptic. Volume transfers with serological pipettes and micropipettors. Become familiar with different forms of basic growth media learn how to transfer a pure bacterial culture from one growth media to another, a. Pdf semesterlong assessment of aseptic technique in. The most important guidelines for pharmaceutical microbiology are described in ich q9. There are also tables of the available products, stability considerations and health and safety issues. An introduction to microbiology, aseptic technique and safety. General aseptic techniques in microbiology laboratory. Learn about aseptic technique, what are the key steps of aseptic technique. May 31, 2012 aseptic technique refers to a set of routine procedures done to prevent sterile solutions and cultures from becoming contaminated by unwanted microorganisms in the laboratory. Lammerts approach is visual and incorporates voice balloons that keep the student focused on the process described. Our new crystalgraphics chart and diagram slides for powerpoint is a collection of over impressively designed datadriven chart and editable diagram s guaranteed to impress any audience.
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